acumen microplate cytometer Search Results


86
SPT Labtech acumen ex3 microplate cytometer
Acumen Ex3 Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acumen ex3 microplate cytometer - by Bioz Stars, 2026-05
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SPT Labtech acumen ex3 laser scanning fluorescence microplate cytometer
Acumen Ex3 Laser Scanning Fluorescence Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen ex3 laser scanning fluorescence microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen ex3 laser scanning fluorescence microplate cytometer - by Bioz Stars, 2026-05
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SPT Labtech acumen explorer laser scanning fluorescence microplate cytometer
Acumen Explorer Laser Scanning Fluorescence Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen explorer laser scanning fluorescence microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen explorer laser scanning fluorescence microplate cytometer - by Bioz Stars, 2026-05
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SPT Labtech acumen explorer ex3 laser scanning microplate cytometer
Acumen Explorer Ex3 Laser Scanning Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen explorer ex3 laser scanning microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen explorer ex3 laser scanning microplate cytometer - by Bioz Stars, 2026-05
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SPT Labtech acumen explorer
Acumen Explorer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen explorer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen explorer - by Bioz Stars, 2026-05
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86
SPT Labtech acumen e x3 microplate cytometer
EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
Acumen E X3 Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen e x3 microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen e x3 microplate cytometer - by Bioz Stars, 2026-05
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SPT Labtech acumen explorer microplate cytometer ex3 hcs
EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
Acumen Explorer Microplate Cytometer Ex3 Hcs, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen explorer microplate cytometer ex3 hcs/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen explorer microplate cytometer ex3 hcs - by Bioz Stars, 2026-05
86/100 stars
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86
SPT Labtech laser scanning fluorescence microplate cytometer
EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
Laser Scanning Fluorescence Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/laser scanning fluorescence microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
laser scanning fluorescence microplate cytometer - by Bioz Stars, 2026-05
86/100 stars
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SPT Labtech acumen microplate cytometer
EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
Acumen Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acumen microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
acumen microplate cytometer - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier

86
SPT Labtech microplate cytometer
EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the <t>Acumen</t> <t>e</t> <t>X3</t> <t>microplate</t> cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
Microplate Cytometer, supplied by SPT Labtech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microplate cytometer/product/SPT Labtech
Average 86 stars, based on 1 article reviews
microplate cytometer - by Bioz Stars, 2026-05
86/100 stars
  Buy from Supplier

Image Search Results


EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).

Journal: British Journal of Cancer

Article Title: Surface proteomic analysis of osteosarcoma identifies EPHA2 as receptor for targeted drug delivery

doi: 10.1038/bjc.2013.578

Figure Lengend Snippet: EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).

Article Snippet: Transduction efficiency was analysed after 48 h by fluorescence read-out using an Acumen e X3 microplate cytometer (TTP LabTech Ltd, Melbourn, UK).

Techniques: Plasmid Preparation, Fluorescence, Transduction, Cytometry, Staining, Expressing, Incubation, Blocking Assay