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Image Search Results
Journal: British Journal of Cancer
Article Title: Surface proteomic analysis of osteosarcoma identifies EPHA2 as receptor for targeted drug delivery
doi: 10.1038/bjc.2013.578
Figure Lengend Snippet: EPHA2 receptor specifically mediates targeted adenoviral vector internalisation into OS cells. ( A ) Representative fluorescence images of SaOS-2 and MG-63 OS cells and Hum63 hp-OBs subjected to transduction with AdYSA at the indicated MOIs, acquired using the Acumen e X3 microplate cytometer. Composite images show Hoechst-stained cell nuclei (blue) and GFP-expression (green). ( B ) Transduction of OS cell lines (green bars) and hp-OBs (blue bars) with EPHA2-targeted adenoviral vector AdYSA, as measured by GFP expression 48 h after subjecting the cells to virus at MOI-100. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. The observed difference between OS cells and hp-OBs is three- to six-fold (Student's t -test; * P <0.05). ( C ) Competition by pre-incubation of the cells with synthetic peptides before incubation with AdYSA. Bars represent mean results of an experiment performed in triplicate; error bars indicate s.d. Green bars represent the control condition in which the cells were incubated with AdYSA alone, grey bars represent the control condition in which cells were pre-incubated with Cys.S peptide, followed by incubation with AdYSA and blue bars represent the competition condition in which cells were pre-incubated with YSA peptide followed by incubation with AdYSA. Receptor blocking with YSA peptide results in significant reduction of transduction efficiency in the OS cell lines (**** P <0.001 for SaOS-2; *** P <0.005 for LM7 and U2OS; ** P <0.01 for MG-63), but not in the already poorly transduced healthy bone cells (NS).
Article Snippet: Transduction efficiency was analysed after 48 h by fluorescence read-out using an
Techniques: Plasmid Preparation, Fluorescence, Transduction, Cytometry, Staining, Expressing, Incubation, Blocking Assay